Hundreds of families heeded the call of disaster coordination authorities to evacuate 10 communities, piling into yellow school buses for trips to shelters.
Nearly 4,000 people have been evacuated from the slopes of the Fuego volcano in Guatemala.
Memories are still fresh of the volcano's eruption in June which swept away villages and left almost 200 people dead and over 235 missing.
A spokesman for Guatemala's disaster management agency CONRED, David de Leon, said 214 residents who live on the slopes of Fuego, mostly in the southern municipality of Escuintla, were moved to safe zones and more will follow.
Images of the volcano after midnight showed glowing lava at the summit.
Soledad Fandiño tiene nuevo novio
Carolina "Pampita" Ardohain concluyó que la coreo sacó el lado sensual de Soledad, y que fue "divertida". Aun así, el periodista de espectáculos no le creyó y la tildó de "ocultadora serial de relaciones".
The volcano is located among Escuintla, Chimaltenango and Sacatepequez in the south-central part of the country.
The Volcano of Fire is one of the most active in Central America.
In June, its explosive flows killed 194 people and left at least 234 missing, although organisations supporting nearby communities insist there are thousands missing.
The stratovolcano southwest of the city of Antigua began erupting on Sunday, with ash and lava spewing from its crater.
About 4000 residents fled Guatemala's Volcano of Fire on Monday (Tuesday NZ Time) as red-hot rock and ash spewed into the sky and cascaded down the slopes toward an area devastated by a deadly eruption earlier this year.